Quantitative polymerase chain reaction (qPCR) is a molecular technique that has reshaped the landscape of biological research and medicine due to its high sensitivity and specificity. qPCR, also known ...
Quantitative PCR (qPCR) has become a cornerstone of molecular biology, offering precise DNA quantification in real time. Yet, challenges such as the need for high-quality nucleic acids, throughput ...
1. To minimize the potential for sample cross-contamination and nucleic acid carryover from one experiment to the next, use aerosol-resistant pipette tips and designated work areas and pipettes for ...
The MIQE guidelines and papers such as the one discussed here can be applied to avoid pitfalls. Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) is everywhere. Its ubiquity, ...
Comparison of expression data requires normalization. The optimum normalization method depends on sample type, with the most common being to normalize to reference genes. It is critical to select ...
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