This lab includes a demonstration of the proper use of a burette system for titrations; including a detailed description, instructional video, and opportunity for students to collect data to ...

All biochemical reactions involve recognition, binding, and the formation of noncovalent complexes. Many small molecule drugs act by selectively binding to protein or nucleic acid targets and altering ...

In this segment, the students discuss the data from their titration lab. In this segment, the students discuss the data from their titration lab. Our host explains the importance of titration in real ...

ANTIBODY TITRATION is critical to the success of any flow cytometry experiment. This is even more so in microflow cytometric analysis of extracellular vesicles (EVs), where removing excess antibodies ...

Everyone that uses titration in their lab knows how simple and fast the technique can be. However, uncertainty around when to replace electrodes creates confusion. Reagent quality, tubing connections ...

DNase footprinting was originally developed as a means to identify where a protein might bind on a DNA sequence 1. The basis for this approach is shown in Figure 1. A DNA fragment, when exposed to ...

Keep the staining reactions at as low a volume as possible to conserve reagents. For instance, you can add 25ml of a 2´ stock of stain to 25 ml of a 2´ stock of cells for a total volume of 50ml. All ...

A titration experiment can be carried out to accurately measure the volume of substances that react in chemical reactions. This technique is commonly used in neutralisation reactions and can also be ...